Agrobacterium tumefaciens-mediated transformation of blueberry (Vaccinium corymbosum L.).
Identifieur interne : 000823 ( Main/Exploration ); précédent : 000822; suivant : 000824Agrobacterium tumefaciens-mediated transformation of blueberry (Vaccinium corymbosum L.).
Auteurs : Guo-Qing Song [États-Unis] ; K C SinkSource :
- Plant cell reports [ 0721-7714 ] ; 2004.
Descripteurs français
- KwdFr :
- ADN des plantes (génétique), Agriculture (méthodes), Agrobacterium tumefaciens (génétique), Clones cellulaires (métabolisme), Glucuronidase (génétique), Gènes rapporteurs (génétique), Génie génétique (méthodes), Génome (MeSH), Pousses de plante (croissance et développement), Pousses de plante (génétique), Pousses de plante (métabolisme), Régions promotrices (génétique) (génétique), Régulation de l'expression des gènes végétaux (génétique), Transformation génétique (génétique), Vaccinium (croissance et développement), Vaccinium (génétique), Vaccinium (métabolisme), Vecteurs génétiques (génétique), Végétaux génétiquement modifiés (croissance et développement), Végétaux génétiquement modifiés (génétique), Végétaux génétiquement modifiés (métabolisme).
- MESH :
- croissance et développement : Pousses de plante, Vaccinium, Végétaux génétiquement modifiés.
- génétique : ADN des plantes, Agrobacterium tumefaciens, Glucuronidase, Gènes rapporteurs, Pousses de plante, Régions promotrices (génétique), Régulation de l'expression des gènes végétaux, Transformation génétique, Vaccinium, Vecteurs génétiques, Végétaux génétiquement modifiés.
- métabolisme : Clones cellulaires, Pousses de plante, Vaccinium, Végétaux génétiquement modifiés.
- méthodes : Agriculture, Génie génétique.
- Génome.
English descriptors
- KwdEn :
- Agriculture (methods), Agrobacterium tumefaciens (genetics), Clone Cells (metabolism), DNA, Plant (genetics), Gene Expression Regulation, Plant (genetics), Genes, Reporter (genetics), Genetic Engineering (methods), Genetic Vectors (genetics), Genome (MeSH), Glucuronidase (genetics), Plant Shoots (genetics), Plant Shoots (growth & development), Plant Shoots (metabolism), Plants, Genetically Modified (genetics), Plants, Genetically Modified (growth & development), Plants, Genetically Modified (metabolism), Promoter Regions, Genetic (genetics), Transformation, Genetic (genetics), Vaccinium (genetics), Vaccinium (growth & development), Vaccinium (metabolism).
- MESH :
- chemical , genetics : DNA, Plant, Glucuronidase.
- genetics : Agrobacterium tumefaciens, Gene Expression Regulation, Plant, Genes, Reporter, Genetic Vectors, Plant Shoots, Plants, Genetically Modified, Promoter Regions, Genetic, Transformation, Genetic, Vaccinium.
- growth & development : Plant Shoots, Plants, Genetically Modified, Vaccinium.
- metabolism : Clone Cells, Plant Shoots, Plants, Genetically Modified, Vaccinium.
- methods : Agriculture, Genetic Engineering.
- Genome.
Abstract
Transient expression studies using blueberry leaf explants and monitored by beta-glucuronidase (GUS) assays indicated Agrobacterium tumefaciens strain EHA105 was more effective than LBA4404 or GV3101; and the use of acetosyringone (AS) at 100 microM for inoculation and 6 days co-cultivation was optimum compared to 2, 4, 8, 10 or 12 days. Subsequently, explants of the cultivars Aurora, Bluecrop, Brigitta, and Legacy were inoculated with strain EHA105 containing the binary vector pBISN1 with the neomycin phosphotransferase gene (nptII) and an intron-interrupted GUS gene directed by the chimeric super promoter (Aocs)3AmasPmas. Co-cultivation was for 6 days on modified woody plant medium (WPM) plus 100 microM AS. Explants were then placed on modified WPM supplemented with 1.0 mg l(-1) thidiazuron, 0.5 mg l(-1) alpha-naphthaleneacetic, 10 mg l(-1) kanamycin (Km), and 250 mg l(-1) cefotaxime. Selection for Km-resistant shoots was carried out in the dark for 2 weeks followed by culture in the light at 30 microE m(-2) s(-1) at 25 degrees C. After 12 weeks, selected shoots that were both Km resistant and GUS positive were obtained from 15.3% of the inoculated leaf explants of cultivar Aurora. Sixty-eight independent clones derived from such shoots all tested positive by the polymerase chain reaction using a nptII primer. Eight of eight among these 68 clones tested positive by Southern hybridization using a gusA gene derived probe. The transformation protocol also yielded Km-resistant, GUS-positive shoots that were also PCR positive at frequencies of 5.0% for Bluecrop, 10.0% for Brigitta and 5.6% for Legacy.
DOI: 10.1007/s00299-004-0842-7
PubMed: 15300402
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Expression Regulation, Plant (genetics)</term><term>Genes, Reporter (genetics)</term><term>Genetic Engineering (methods)</term><term>Genetic Vectors (genetics)</term><term>Genome (MeSH)</term><term>Glucuronidase (genetics)</term><term>Plant Shoots (genetics)</term><term>Plant Shoots (growth & development)</term><term>Plant Shoots (metabolism)</term><term>Plants, Genetically Modified (genetics)</term><term>Plants, Genetically Modified (growth & development)</term><term>Plants, Genetically Modified (metabolism)</term><term>Promoter Regions, Genetic (genetics)</term><term>Transformation, Genetic (genetics)</term><term>Vaccinium (genetics)</term><term>Vaccinium (growth & development)</term><term>Vaccinium (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN des plantes (génétique)</term><term>Agriculture (méthodes)</term><term>Agrobacterium tumefaciens (génétique)</term><term>Clones cellulaires (métabolisme)</term><term>Glucuronidase (génétique)</term><term>Gènes 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xml:lang="fr"><term>Pousses de plante</term><term>Vaccinium</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Agrobacterium tumefaciens</term><term>Gene Expression Regulation, Plant</term><term>Genes, Reporter</term><term>Genetic Vectors</term><term>Plant Shoots</term><term>Plants, Genetically Modified</term><term>Promoter Regions, Genetic</term><term>Transformation, Genetic</term><term>Vaccinium</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Plant Shoots</term><term>Plants, Genetically Modified</term><term>Vaccinium</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN des plantes</term><term>Agrobacterium tumefaciens</term><term>Glucuronidase</term><term>Gènes rapporteurs</term><term>Pousses de plante</term><term>Régions promotrices (génétique)</term><term>Régulation de l'expression des gènes végétaux</term><term>Transformation 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using blueberry leaf explants and monitored by beta-glucuronidase (GUS) assays indicated Agrobacterium tumefaciens strain EHA105 was more effective than LBA4404 or GV3101; and the use of acetosyringone (AS) at 100 microM for inoculation and 6 days co-cultivation was optimum compared to 2, 4, 8, 10 or 12 days. Subsequently, explants of the cultivars Aurora, Bluecrop, Brigitta, and Legacy were inoculated with strain EHA105 containing the binary vector pBISN1 with the neomycin phosphotransferase gene (nptII) and an intron-interrupted GUS gene directed by the chimeric super promoter (Aocs)3AmasPmas. Co-cultivation was for 6 days on modified woody plant medium (WPM) plus 100 microM AS. Explants were then placed on modified WPM supplemented with 1.0 mg l(-1) thidiazuron, 0.5 mg l(-1) alpha-naphthaleneacetic, 10 mg l(-1) kanamycin (Km), and 250 mg l(-1) cefotaxime. Selection for Km-resistant shoots was carried out in the dark for 2 weeks followed by culture in the light at 30 microE m(-2) s(-1) at 25 degrees C. After 12 weeks, selected shoots that were both Km resistant and GUS positive were obtained from 15.3% of the inoculated leaf explants of cultivar Aurora. Sixty-eight independent clones derived from such shoots all tested positive by the polymerase chain reaction using a nptII primer. Eight of eight among these 68 clones tested positive by Southern hybridization using a gusA gene derived probe. The transformation protocol also yielded Km-resistant, GUS-positive shoots that were also PCR positive at frequencies of 5.0% for Bluecrop, 10.0% for Brigitta and 5.6% for Legacy.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15300402</PMID><DateCompleted><Year>2005</Year><Month>12</Month><Day>23</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0721-7714</ISSN><JournalIssue CitedMedium="Print"><Volume>23</Volume><Issue>7</Issue><PubDate><Year>2004</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Plant cell reports</Title><ISOAbbreviation>Plant Cell Rep</ISOAbbreviation></Journal><ArticleTitle>Agrobacterium tumefaciens-mediated transformation of blueberry (Vaccinium corymbosum L.).</ArticleTitle><Pagination><MedlinePgn>475-84</MedlinePgn></Pagination><Abstract><AbstractText>Transient expression studies using blueberry leaf explants and monitored by beta-glucuronidase (GUS) assays indicated Agrobacterium tumefaciens strain EHA105 was more effective than LBA4404 or GV3101; and the use of acetosyringone (AS) at 100 microM for inoculation and 6 days co-cultivation was optimum compared to 2, 4, 8, 10 or 12 days. Subsequently, explants of the cultivars Aurora, Bluecrop, Brigitta, and Legacy were inoculated with strain EHA105 containing the binary vector pBISN1 with the neomycin phosphotransferase gene (nptII) and an intron-interrupted GUS gene directed by the chimeric super promoter (Aocs)3AmasPmas. Co-cultivation was for 6 days on modified woody plant medium (WPM) plus 100 microM AS. Explants were then placed on modified WPM supplemented with 1.0 mg l(-1) thidiazuron, 0.5 mg l(-1) alpha-naphthaleneacetic, 10 mg l(-1) kanamycin (Km), and 250 mg l(-1) cefotaxime. Selection for Km-resistant shoots was carried out in the dark for 2 weeks followed by culture in the light at 30 microE m(-2) s(-1) at 25 degrees C. After 12 weeks, selected shoots that were both Km resistant and GUS positive were obtained from 15.3% of the inoculated leaf explants of cultivar Aurora. Sixty-eight independent clones derived from such shoots all tested positive by the polymerase chain reaction using a nptII primer. Eight of eight among these 68 clones tested positive by Southern hybridization using a gusA gene derived probe. The transformation protocol also yielded Km-resistant, GUS-positive shoots that were also PCR positive at frequencies of 5.0% for Bluecrop, 10.0% for Brigitta and 5.6% for Legacy.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Song</LastName><ForeName>Guo-Qing</ForeName><Initials>GQ</Initials><AffiliationInfo><Affiliation>Plant Transformation Center, Department of Horticulture, Michigan State University, East Lansing, MI, 48824, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sink</LastName><ForeName>K C</ForeName><Initials>KC</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2004</Year><Month>08</Month><Day>05</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Plant Cell Rep</MedlineTA><NlmUniqueID>9880970</NlmUniqueID><ISSNLinking>0721-7714</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018744">DNA, Plant</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.31</RegistryNumber><NameOfSubstance UI="D005966">Glucuronidase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000383" MajorTopicYN="N">Agriculture</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016960" MajorTopicYN="N">Agrobacterium tumefaciens</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002999" MajorTopicYN="N">Clone Cells</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018744" MajorTopicYN="N">DNA, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017930" MajorTopicYN="N">Genes, Reporter</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005818" MajorTopicYN="N">Genetic Engineering</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005822" MajorTopicYN="N">Genetic Vectors</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016678" MajorTopicYN="N">Genome</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005966" MajorTopicYN="N">Glucuronidase</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018520" MajorTopicYN="N">Plant Shoots</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011401" MajorTopicYN="N">Promoter Regions, Genetic</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014170" MajorTopicYN="N">Transformation, Genetic</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D029796" MajorTopicYN="N">Vaccinium</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2004</Year><Month>02</Month><Day>02</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2004</Year><Month>06</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2004</Year><Month>06</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2004</Year><Month>8</Month><Day>10</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2005</Year><Month>12</Month><Day>24</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2004</Year><Month>8</Month><Day>10</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15300402</ArticleId><ArticleId IdType="doi">10.1007/s00299-004-0842-7</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Plant Cell Rep. 1995 Jan;15(1-2):82-6</Citation><ArticleIdList><ArticleId IdType="pubmed">24185660</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Mol Biol. 1992 Dec;20(6):1037-48</Citation><ArticleIdList><ArticleId IdType="pubmed">1463839</ArticleId></ArticleIdList></Reference><Reference><Citation>EMBO J. 1987 Dec 20;6(13):3901-7</Citation><ArticleIdList><ArticleId IdType="pubmed">3327686</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 1996 May;8(5):873-86</Citation><ArticleIdList><ArticleId IdType="pubmed">8672885</ArticleId></ArticleIdList></Reference><Reference><Citation>J Agric Food Chem. 2001 May;49(5):2222-7</Citation><ArticleIdList><ArticleId IdType="pubmed">11368580</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 1985 Mar 8;227(4691):1229-31</Citation><ArticleIdList><ArticleId IdType="pubmed">17757866</ArticleId></ArticleIdList></Reference><Reference><Citation>J Gen Virol. 1975 Jan;26(1):33-48</Citation><ArticleIdList><ArticleId IdType="pubmed">1123610</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Michigan</li></region><settlement><li>East Lansing</li></settlement><orgName><li>Université d'État du Michigan</li></orgName></list><tree><noCountry><name sortKey="Sink, K C" sort="Sink, K C" uniqKey="Sink K" first="K C" last="Sink">K C Sink</name></noCountry><country name="États-Unis"><region name="Michigan"><name sortKey="Song, Guo Qing" sort="Song, Guo Qing" uniqKey="Song G" first="Guo-Qing" last="Song">Guo-Qing Song</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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